Dhoot G K, Perry S V
Exp Neurol. 1983 Oct;82(1):131-42. doi: 10.1016/0014-4886(83)90248-0.
In the newborn rat all cells of soleus, extensor digitorum longus (EDL), and tibialis anterior (TA) muscles stained for fast troponin I. A proportion of the cells, that was much higher in the soleus, also stained for slow troponin I. Fast and slow troponin I were segregated in different cell types in all three muscles 10 to 12 days after birth. No subsequent changes in the distribution of the two forms of troponin I occurred with further growth of EDL and TA muscles. The number of type I cells in soleus steadily increased with increasing age to 24 weeks. Three weeks after denervation at birth, almost all cells in soleus muscle stained for fast troponin I but less than 5% stained significantly dark for slow troponin I. All cells stained for myosin ATPase after alkaline preincubation, but very few after acid preincubation. Three weeks after denervation of EDL and to a lesser extent with TA muscle, fast and slow troponin I were still segregated in different cells. After alkaline preincubation all cells stained equally dark for myosin ATPase but only those positive for slow troponin I stained for myosin ATPase after acid preincubation.
