Maruyama T, Makino T, Sugi T, Matsubayashi H, Ozawa N, Nozawa S
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Hum Immunol. 1992 Jul;34(3):212-8. doi: 10.1016/0198-8859(92)90114-3.
We undertook an investigation in which flow cytometry was used to characterize immune cell populations in the decidua of first-trimester normal pregnancies, spontaneous abortions, and ectopic pregnancies in comparison to the nonpregnant endometrium to demonstrate how the proportions of immunocompetent cell populations at the fetomaternal interface are influenced by the presence and state of a fetoplacental allograft. No significant differences were found in the decidua of the different types of first-trimester pregnancy in the proportions of the CD45+, CD14+, CD3+, CD4+, CD8+, CD19+, CD3-/CD16+ and/or CD56+, CD3+/CD16+ and/or CD56+, CD4+/Leu-8+, CD4+/Leu-8-, CD8+/CD11b+, CD8+/CD11b-, and CD3+/HLA-DR- decidual leukocyte subsets. However, the percentage of decidual CD3+/HLA-DR+ cells, which are characteristic of activated T cells, was significantly higher in spontaneous abortions than in normal pregnancies (p less than 0.05). This suggests that the accumulation of decidual leukocytes may be regulated mainly by hormones and/or cytokines rather than by the presence and state of an intrauterine conceptus and that on/off-switching of activation of decidual T cells may be associated with successful maintenance of the implanted embryo.
我们进行了一项研究，采用流式细胞术对孕早期正常妊娠、自然流产和异位妊娠的蜕膜中的免疫细胞群体进行表征，并与非妊娠子宫内膜进行比较，以证明胎儿-胎盘同种异体移植物的存在和状态如何影响母胎界面处免疫活性细胞群体的比例。在不同类型的孕早期妊娠的蜕膜中，CD45+、CD14+、CD3+、CD4+、CD8+、CD19+、CD3-/CD16+和/或CD56+、CD3+/CD16+和/或CD56+、CD4+/Leu-8+、CD4+/Leu-8-、CD8+/CD11b+、CD8+/CD11b-以及CD3+/HLA-DR-蜕膜白细胞亚群的比例未发现显著差异。然而，作为活化T细胞特征的蜕膜CD3+/HLA-DR+细胞百分比在自然流产中显著高于正常妊娠（p小于0.05）。这表明蜕膜白细胞的积累可能主要受激素和/或细胞因子调节，而非受子宫内孕体的存在和状态影响，并且蜕膜T细胞活化的开启/关闭可能与植入胚胎的成功维持有关。
