Vassiliadou N, Bulmer J N
Department of Pathology, University of Newcastle, Royal Victoria Infirmary, Newcastle upon Tyne, UK.
Immunology. 1998 Jul;94(3):368-75. doi: 10.1046/j.1365-2567.1998.00497.x.
Flow cytometry studies of first-trimester normal human decidua have proposed that a proportion of endometrial granulated lymphocytes (eGL), the predominant leucocyte population in early human pregnancy, co-express CD69. The purpose of this study was to investigate CD69 expression by immunohistochemistry throughout the menstrual cycle and in first-trimester human decidua and to determine whether CD69 expression by eGL is an in vivo characteristic or whether the antigen is acquired in vitro after tissue disaggregation and cell purification. Single immunoenzymatic and double immunofluorescence labelling of tissue sections, supplemented with double immunoenzymatic staining of purified eGL and flow cytometry, indicated that eGL do not co-express CD69 in situ but that they acquire the molecule in vitro after cell purification and culture.
对孕早期正常人类蜕膜进行的流式细胞术研究表明，一部分子宫内膜颗粒淋巴细胞（eGL），即人类妊娠早期主要的白细胞群体，共表达CD69。本研究的目的是通过免疫组织化学研究整个月经周期以及孕早期人类蜕膜中CD69的表达情况，并确定eGL表达CD69是一种体内特征，还是该抗原在组织解离和细胞纯化后于体外获得。对组织切片进行单免疫酶标和双免疫荧光标记，并辅以对纯化的eGL进行双免疫酶标染色和流式细胞术检测，结果表明eGL在原位并不共表达CD69，但在细胞纯化和培养后于体外获得该分子。
