Ho H N, Chao K H, Chen C K, Yang Y S, Huang S C
Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, ROC.
Hum Immunol. 1996 Sep 1;49(2):130-6. doi: 10.1016/0198-8859(96)00120-6.
Endometrial lymphocytes were studied at all stages throughout the menstrual cycle and early pregnancy by flow cytometry to examine different lymphocyte subpopulations and the expression of the T- and NK-cell activation markers. After pregnancy, CD8+CD3+ lymphocytes were decreased in the decidua. In both endometrium and decidua, more T cells expressed CD69, CD71, HLA-DR, and CD38 antigens than in peripheral blood. After pregnancy, CD71+CD3+ lymphocytes were further increased. CD25+CD3+ lymphocytes decreased significantly in the endometrium and decidua of ectopic pregnancies, but not in the decidua of normal pregnancies. These findings indicate that T cells are regionally activated in the first trimester, and it may be the result of the stimulation by fetal antigens. NK cells were the most abundant cell type in the decidua, which expressed the phenotype CD16- CD56+, and CD57-CD56+. The proportion of activated decidual NK cells was increased in anembryonic pregnancies more than in normal pregnancies, although the total NK subpopulation was similar in both groups. This might result in increased NK cytotoxicity in anembryonic pregnancies. In conclusion, T cells are activated, but NK cytotoxicity is decreased in the decidua of early normal pregnancies. This might be important in the control of trophoblast growth and placental development.
通过流式细胞术研究了整个月经周期及妊娠早期各阶段的子宫内膜淋巴细胞，以检测不同淋巴细胞亚群以及T细胞和NK细胞活化标志物的表达。妊娠后，蜕膜中CD8⁺CD3⁺淋巴细胞减少。与外周血相比，子宫内膜和蜕膜中表达CD69、CD71、HLA - DR和CD38抗原的T细胞更多。妊娠后，CD71⁺CD3⁺淋巴细胞进一步增加。异位妊娠的子宫内膜和蜕膜中CD25⁺CD3⁺淋巴细胞显著减少，但正常妊娠的蜕膜中无此现象。这些发现表明，孕早期T细胞在局部被激活，这可能是胎儿抗原刺激的结果。NK细胞是蜕膜中最丰富的细胞类型，其表型为CD16⁻CD56⁺和CD57⁻CD56⁺。尽管两组的NK亚群总数相似，但空孕囊妊娠中活化的蜕膜NK细胞比例比正常妊娠增加得更多。这可能导致空孕囊妊娠中NK细胞毒性增加。总之，在早期正常妊娠的蜕膜中T细胞被激活，但NK细胞毒性降低。这可能对滋养层生长和胎盘发育的调控具有重要意义。
