在八个新建立的HTLV-I阳性T细胞系中T细胞和髓样细胞表型的同时表达。
Simultaneous expression of T-cell and myeloid cell phenotypes in eight newly established HTLV-I-positive T-cell lines.
作者信息
Koizumi S, Sugiura M, Zhang X K, Yamashiro K, Iwanaga M, Imai S, Osato T
机构信息
Department of Virology, Hokkaido University School of Medicine, Sapporo.
出版信息
Jpn J Cancer Res. 1992 Sep;83(9):929-32. doi: 10.1111/j.1349-7006.1992.tb02002.x.
Eight cell lines were established from patients with adult T-cell leukemia, and from normal adults, by cocultivation with human T-cell leukemia virus type I(HTLV-I)-producer cell lines in the presence of interleukin-2. All of these cell lines harbored HTLV-I and showed T-cell markers CD2, CD3 and CD4, but not B-cell markers. Unexpectedly, all eight cell lines expressed a myeloid marker CD13 and three of the eight lines also expressed another myeloid marker CD33. Dual staining showed the simultaneous expression of CD3 and CD13 on the same cells. Thus, evidence was obtained for the expression of myeloid antigens on HTLV-I-harboring T cells.
通过在白细胞介素-2存在的情况下与I型人类T细胞白血病病毒（HTLV-I）产生细胞系共培养，从成人T细胞白血病患者和正常成年人中建立了8个细胞系。所有这些细胞系都含有HTLV-I，并显示T细胞标志物CD2、CD3和CD4，但不显示B细胞标志物。出乎意料的是，所有8个细胞系都表达髓系标志物CD13，其中3个细胞系还表达另一种髓系标志物CD33。双重染色显示同一细胞上同时表达CD3和CD13。因此，获得了HTLV-I携带T细胞上髓系抗原表达的证据。
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