从患有I型人类嗜T淋巴细胞病毒相关脊髓病（HAM）患者的脑脊液中建立的人T细胞系。

Human T cell lines established from the cerebrospinal fluid of patients with human T lymphotropic virus type I-associated myelopathy (HAM).

作者信息

Nishimura M, Akiguchi I, Takigawa M, Fujita M, Kameyama M, Maeda M

机构信息

Department of Neurology, Faculty of Medicine, Kyoto University, Japan.

出版信息

J Neuroimmunol. 1988 Feb;17(3):229-36. doi: 10.1016/0165-5728(88)90071-9.

DOI:10.1016/0165-5728(88)90071-9

PMID:2892858

Abstract

T cell lines were established from the cerebrospinal fluid (CSF) lymphocytes of two patients with human T lymphotropic virus type I (HTLV-I)-associated myelopathy (HAM). These two interleukin-2 (IL-2)-dependent T cell lines have been cultured for more than 8 months without any accessory cells. The surface phenotype of these cells was CD2(+), CD4(+), CD8(-), Ia(+) and Tac(+). Southern blot hybridization analysis revealed the presence of HTLV-I provirus in these cells and C-type retrovirus particles were identified by electron microscopy. These findings indicate the presence of HTLV-I infected helper T lymphocytes in the CSF of the patients with HAM. These HTLV-I(+) T cell lines may be valuable for investigating the possible neutrotropism of HTLV-I and the role of HTLV-I in the pathogenesis of HAM.

摘要

从两名成人T细胞白血病病毒I型（HTLV-I）相关脊髓病（HAM）患者的脑脊液（CSF）淋巴细胞中建立了T细胞系。这两个依赖白细胞介素2（IL-2）的T细胞系在没有任何辅助细胞的情况下已培养了8个月以上。这些细胞的表面表型为CD2(+)、CD4(+)、CD8(-)、Ia(+)和Tac(+)。Southern印迹杂交分析显示这些细胞中存在HTLV-I前病毒，并且通过电子显微镜鉴定出C型逆转录病毒颗粒。这些发现表明HAM患者的脑脊液中存在HTLV-I感染的辅助性T淋巴细胞。这些HTLV-I(+) T细胞系对于研究HTLV-I可能的嗜中性粒细胞特性以及HTLV-I在HAM发病机制中的作用可能具有重要价值。