棒状杆菌提取物对仲醇的氧化作用
OXIDATION OF SECONDARY ALCOHOLS BY EXTRACTS OF A CORYNEBACTERIUM.
作者信息
ROSENBERG E, HOLMES P
出版信息
J Bacteriol. 1965 May;89(5):1212-6. doi: 10.1128/jb.89.5.1212-1216.1965.
Rosenberg, Eugene (University of California, Los Angeles), and Paul Holmes. Oxidation of secondary alcohols by extracts of a Corynebacterium. J. Bacteriol. 89:1212-1216. 1965.-A Corynebacterium was isolated from soil by use of the enrichment culture technique. This Corynebacterium oxidized and grew on the chemically synthesized secondary alcohol, 3-tetrahydrofuranol. Extracts of this organism contained at least two different nicotinamide adenine dinucleotide-requiring soluble secondary alcohol dehydrogenases. These enzymes were distinguished by the relative rates at which they oxidized thymidylic acid and 3-tetrahydrofuranol. In addition to their substrate specificity, the two enzymes differed in pH optima and thermal stability. Also, the 3-tetrahydrofuranol dehydrogenase was induced by 3-tetrahydrofuranol, whereas the thymidylic acid dehydrogenase was constitutive.
罗森伯格，尤金（加利福尼亚大学洛杉矶分校）和保罗·霍姆斯。棒状杆菌提取物对仲醇的氧化作用。《细菌学杂志》89:1212 - 1216。1965年。——通过富集培养技术从土壤中分离出一种棒状杆菌。这种棒状杆菌能氧化化学合成的仲醇3 - 四氢呋喃醇并在其上生长。该生物体的提取物含有至少两种不同的需要烟酰胺腺嘌呤二核苷酸的可溶性仲醇脱氢酶。这些酶通过它们氧化胸苷酸和3 - 四氢呋喃醇的相对速率来区分。除了底物特异性外，这两种酶在最适pH值和热稳定性方面也有所不同。此外，3 - 四氢呋喃醇脱氢酶由3 - 四氢呋喃醇诱导产生，而胸苷酸脱氢酶是组成型的。
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