一种禽类抗原降解酶的制备与性质
PREPARATION AND PROPERTIES OF A VI ANTIGEN-DEGRADING ENZYME.
作者信息
BAKER E E, WHITESIDE R E
出版信息
J Bacteriol. 1965 May;89(5):1217-24. doi: 10.1128/jb.89.5.1217-1224.1965.
Baker, Edgar E. (Boston University School of Medicine, Boston, Mass.), and Roberta E. Whiteside. Preparation and properties of a Vi antigen-degrading enzyme. J. Bacteriol. 89:1217-1224. 1965.-Vi antigen can be hydrolyzed by an inducible enzyme produced by a microorganism isolated from soil. The organism can be identified as Bacillus sphaericus. The enzyme in the culture supernatant fluid can be concentrated and partially purified by precipitation with ammonium sulfate at 50% of saturation. The partially purified enzyme is relatively stable. Incubation of the enzyme with Vi antigen causes a rapid loss of serological activity, a decrease in viscosity, the loss of ability to precipitate albumin at pH 4.0, and the production of reducing sugar and hexosamine. Optimal conditions for enzyme action on Vi antigen are pH 8.4 and 40 C. The reaction rate is independent of the substrate concentration. Intact protein and calcium activates the Vi antigen-enzyme system. The enzyme is not inhibited by its reaction products.
贝克，埃德加·E.（波士顿大学医学院，马萨诸塞州波士顿），以及罗伯塔·E.怀特赛德。Vi抗原降解酶的制备及性质。《细菌学杂志》89:1217 - 1224。1965年。- Vi抗原可被从土壤中分离出的一种微生物产生的诱导酶水解。该微生物可鉴定为球形芽孢杆菌。培养上清液中的酶可通过用50%饱和度的硫酸铵沉淀进行浓缩和部分纯化。部分纯化的酶相对稳定。酶与Vi抗原一起温育会导致血清学活性迅速丧失、粘度降低、在pH 4.0时沉淀白蛋白的能力丧失，以及还原糖和己糖胺的产生。酶作用于Vi抗原的最佳条件是pH 8.4和40℃。反应速率与底物浓度无关。完整的蛋白质和钙可激活Vi抗原 - 酶系统。该酶不受其反应产物的抑制。
Zotero 插件