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与伤寒沙门氏菌Vi抗原发生交叉反应的大肠杆菌B多糖的纯化及免疫化学特性:该多糖与大肠杆菌K1抗原发生交叉反应的初步证据

Purification and immunochemical properties of Escherichia coli B polysaccharide cross-reacting with Salmonella typhi Vi antigen: preliminary evidence for cross-reaction of the polysaccharide with Escherichia coli K1 antigen.

作者信息

Szewczyk B, Taylor A

出版信息

Infect Immun. 1983 Jul;41(1):224-31. doi: 10.1128/iai.41.1.224-231.1983.

Abstract

An acidic polysaccharide of Escherichia coli B was isolated by a mild procedure and purified to homogeneity. The polysaccharide was found to react in Salmonella typhi Vi antisera and E. coli K1 antisera. Serological analysis and preliminary chemical characterization of the polysaccharide indicated that it is an aminouronic acid polymer which, although not structurally identical to either Vi or K1, appears more like the Vi antigen, both immunochemically and chemically.

摘要

通过温和的方法从大肠杆菌B中分离出一种酸性多糖,并将其纯化至同质。发现该多糖能与伤寒沙门氏菌Vi抗血清和大肠杆菌K1抗血清发生反应。对该多糖的血清学分析和初步化学表征表明,它是一种氨基糖醛酸聚合物,虽然在结构上与Vi或K1都不相同,但在免疫化学和化学方面似乎更类似于Vi抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/9ab02927e34d/iai00136-0236-a.jpg

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本文引用的文献

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ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS.抗原-抗体交叉电泳
Anal Biochem. 1965 Feb;10:358-61. doi: 10.1016/0003-2697(65)90278-2.
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A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
Anal Biochem. 1962 Oct;4:330-4. doi: 10.1016/0003-2697(62)90095-7.

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