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与伤寒沙门氏菌Vi抗原发生交叉反应的大肠杆菌B多糖的纯化及免疫化学特性:该多糖与大肠杆菌K1抗原发生交叉反应的初步证据

Purification and immunochemical properties of Escherichia coli B polysaccharide cross-reacting with Salmonella typhi Vi antigen: preliminary evidence for cross-reaction of the polysaccharide with Escherichia coli K1 antigen.

作者信息

Szewczyk B, Taylor A

出版信息

Infect Immun. 1983 Jul;41(1):224-31. doi: 10.1128/iai.41.1.224-231.1983.

DOI:10.1128/iai.41.1.224-231.1983
PMID:6345392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC264767/
Abstract

An acidic polysaccharide of Escherichia coli B was isolated by a mild procedure and purified to homogeneity. The polysaccharide was found to react in Salmonella typhi Vi antisera and E. coli K1 antisera. Serological analysis and preliminary chemical characterization of the polysaccharide indicated that it is an aminouronic acid polymer which, although not structurally identical to either Vi or K1, appears more like the Vi antigen, both immunochemically and chemically.

摘要

通过温和的方法从大肠杆菌B中分离出一种酸性多糖,并将其纯化至同质。发现该多糖能与伤寒沙门氏菌Vi抗血清和大肠杆菌K1抗血清发生反应。对该多糖的血清学分析和初步化学表征表明,它是一种氨基糖醛酸聚合物,虽然在结构上与Vi或K1都不相同,但在免疫化学和化学方面似乎更类似于Vi抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/d393e39b2064/iai00136-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/9ab02927e34d/iai00136-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/8a7b463c0b11/iai00136-0236-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/d393e39b2064/iai00136-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/9ab02927e34d/iai00136-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/8a7b463c0b11/iai00136-0236-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017b/264767/d393e39b2064/iai00136-0238-a.jpg

相似文献

1
Purification and immunochemical properties of Escherichia coli B polysaccharide cross-reacting with Salmonella typhi Vi antigen: preliminary evidence for cross-reaction of the polysaccharide with Escherichia coli K1 antigen.与伤寒沙门氏菌Vi抗原发生交叉反应的大肠杆菌B多糖的纯化及免疫化学特性:该多糖与大肠杆菌K1抗原发生交叉反应的初步证据
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本文引用的文献

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Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues.组织中酸性多糖测定中的脂肪族铵盐
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ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS.抗原-抗体交叉电泳
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VI-PHAGE RECEPTOR PROPERTIES OF VI-POLYSACCHARIDES ISOLATED BY DIFFERENT METHODS.不同方法分离的Vi-多糖的Vi-噬菌体受体特性
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A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
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Pyruvic acid, a unique component of an exocellular bacterial polysaccharide.丙酮酸,一种胞外细菌多糖的独特成分。
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Sialic acids (N,7-O-diacetylneuraminic acid and N-acetylneuraminic adcid) in Escherichia coli. II. Their presence on the cell wall surface and relationship to K antigen.大肠杆菌中的唾液酸(N,7-O-二乙酰神经氨酸和N-乙酰神经氨酸)。II. 它们在细胞壁表面的存在及其与K抗原的关系。
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Sialic acids (N,7-O-diacetylneuraminic acid and N-acetylneuraminic acid) in Escherichia coli. I. Isolation and identification.大肠杆菌中的唾液酸(N,7-O-二乙酰神经氨酸和N-乙酰神经氨酸)。I. 分离与鉴定。
J Bacteriol. 1961 Dec;82(6):838-48. doi: 10.1128/jb.82.6.838-848.1961.
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A simplified spectrophotometric determination of ester groups in lipids.脂质中酯基的简化分光光度测定法。
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The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
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