Chevalier J, Crémieux A
Laboratoire de Microbiologie, Hygiène Microbienne, Immunologie, Faculté de Pharmacie, Marseille, France.
J Appl Bacteriol. 1992 Oct;73(4):342-8. doi: 10.1111/j.1365-2672.1992.tb04987.x.
Studies were carried out to detect the modifications, if any, on the peri-umbilical flora of six healthy volunteers after two or three daily applications of Hexomedine solution (HEX) and Betadine solution (PVI) repeated for five consecutive days. A standardized scrubbing method was used for bacterial sampling. Surviving bacteria were selected with both selective and non-selective media, and then identified by gas chromatographic fatty acid analysis. Both antiseptics were highly effective, showing both immediate and residual antimicrobial activities. The use of HEX led to a slight increase in Gram-positive cocci and a small decrease in coryneforms, but PVI produced a marked increase in Gram-positive cocci and a sharp decrease in coryneforms. The two antiseptics, however, caused no major alteration in the cutaneous microbial population. Indeed, neither the overgrowth of Gram-negative bacilli nor the emergence of resistant species was observed.
开展了多项研究，以检测连续五天每天两到三次应用己脒定溶液（HEX）和聚维酮碘溶液（PVI）后，六名健康志愿者脐周菌群是否发生变化。采用标准化擦洗方法进行细菌采样。使用选择性和非选择性培养基筛选存活细菌，然后通过气相色谱脂肪酸分析进行鉴定。两种防腐剂均具有高效性，显示出即时和残留抗菌活性。使用HEX导致革兰氏阳性球菌略有增加，棒状杆菌略有减少，但PVI使革兰氏阳性球菌显著增加，棒状杆菌急剧减少。然而，这两种防腐剂并未引起皮肤微生物群落的重大改变。实际上，未观察到革兰氏阴性杆菌过度生长或耐药菌出现。
