类风湿因子与动物γ球蛋白的反应：定量考量

THE REACTION OF RHEUMATOID FACTOR WITH ANIMAL GAMMA-GLOBULINS: QUANTITATIVE CONSIDERATIONS.

作者信息

BUTLER V P, VAUGHAN J H

出版信息

Immunology. 1965 Feb;8(2):144-59.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1423422/

Abstract

Bis-diazotized benzidine has been conjugated to the γ-globulins of three species to produce soluble aggregates and insoluble aggregates for use in the study of the rheumatoid factor. Soluble aggregates have proved useful in the study of the interaction of rheumatoid factor with human and rabbit γ-globulins by agar diffusion methods. Insoluble complexes have provided a method for the absorption and quantitative determination of the rheumatoid factor capable of reacting with the γ-globulins of various species. The rheumatoid factor in three sera extensively studied has been demonstrated to consist of a heterogeneous entity, which reacts with human γ-globulin and moieties of which also react with rabbit, bovine and equine γ-globulins. The extent of cross-reactivity with rabbit and bovine γ-globulins has been demonstrated to differ from one serum to another. The characteristics of the reactivities of rheumatoid factor make it seem most probable that the rheumatoid factor is an antibody to denatured human γ-globulin possessing cross-reactivity with other mammalian γ-globulins.

摘要

双偶氮联苯胺已与三种动物的γ球蛋白结合，以制备可溶性聚集体和不溶性聚集体，用于类风湿因子的研究。通过琼脂扩散法，可溶性聚集体已被证明在研究类风湿因子与人及兔γ球蛋白的相互作用中很有用。不溶性复合物提供了一种吸收和定量测定能够与各种动物γ球蛋白反应的类风湿因子的方法。对三份血清进行广泛研究后发现，类风湿因子是一种异质性物质，它与人γ球蛋白反应，其部分成分也与兔、牛和马的γ球蛋白反应。已证明与兔和牛γ球蛋白的交叉反应程度在不同血清之间存在差异。类风湿因子的反应特性表明，类风湿因子很可能是一种针对变性人γ球蛋白的抗体，与其他哺乳动物γ球蛋白具有交叉反应性。