Normansell D E, Stanworth D R
Immunology. 1968 Oct;15(4):549-60.
Interactions between a rheumatoid factor preparation and native human (normal and myeloma) and animal γG-globulins have been studied in the ultracentrifuge. Using pooled normal γG-globulin or a myeloma γG-globulin, the extent of reaction has been shown to be dependent upon the reactant concentration employed, a four-fold excess, by weight, of γG-globulin over rheumatoid factor being required to ensure maximum production of 22S complex. All native myeloma γG-globulins tested reacted to give a 22S complex, the majority showing similar reactivity to the normal γG-globulin control. A small proportion, however, showed significantly different reactivities. Of the animal γG-globulins tested, only rhesus monkey γG-globulin showed reactivity similar to human γG-globulin. The other species showed decreased reactivity. The importance of these findings is discussed.
已在超速离心机中研究了类风湿因子制剂与天然人（正常和骨髓瘤）及动物γG球蛋白之间的相互作用。使用混合的正常γG球蛋白或骨髓瘤γG球蛋白，已表明反应程度取决于所用反应物的浓度，为确保最大程度地生成22S复合物，γG球蛋白的重量需比类风湿因子过量四倍。所有测试的天然骨髓瘤γG球蛋白都发生反应生成22S复合物，大多数显示出与正常γG球蛋白对照相似的反应性。然而，一小部分显示出明显不同的反应性。在测试的动物γG球蛋白中，只有恒河猴γG球蛋白显示出与人γG球蛋白相似的反应性。其他物种的反应性降低。讨论了这些发现的重要性。
