KORNFELD L, WEIGLE W O
Immunology. 1965 Feb;8(2):213-20.
C′ haemolytic activity and radioactivity were detected in EDTA eluates of immune precipitates formed in the presence of either whole guinea-pig serum or euglobulin fraction labelled with S . Eluted C′ and S were fixed by sensitized sheep cells which subsequently lysed on exposure to guinea-pig C′ reagents lacking C′. The dissociated material contributed only a minute fraction of the protein N taken up by Ag—Ab precipitates from fresh C′.
在存在全豚鼠血清或用 S 标记的优球蛋白组分形成的免疫沉淀物的 EDTA 洗脱液中检测到 C′溶血活性和放射性。洗脱的 C′和 S 被致敏绵羊细胞固定,随后在暴露于缺乏 C′的豚鼠 C′试剂时裂解。解离的物质仅占新鲜 C′中 Ag—Ab 沉淀物摄取的蛋白质 N 的一小部分。