致敏红细胞与补体第一成分之间反应的研究。
Studies of the reaction between the sensitized erythrocyte and the first component of complement.
作者信息
WIRTZ G H, BECKER E L
出版信息
Immunology. 1961 Oct;4(4):473-85.
When sensitized erythrocytes (EA) possessing the 1st (C′), 4th (C′) and 2nd (C′) components of haemolytic complement were treated with 1,4-butanediamine, C′ but not C′ or C′ was removed from the cell. All the straight chain diamines of 3 to 8 carbons, with the amino groups on either end, removed C′ with about equal facility; the diamines were considerably more active than the corresponding monoamines. C′ could be recovered in solution following its removal from the red cell by the diamine. The rate of detachment of C′ was quite high for the first 10 minutes, after which it was slower and followed first order kinetics. It appears that C′ and the diamine compete for the same site on the sensitized erythrocyte. The following observations suggest that Ca has a role in the EAC′ bond: () An increased Ca concentration diminishes the effect of the diamine. () The EAC′ bond dissociates at low bivalent cation concentration. () A number of bivalent cations will preserve the EAC′ bond, Ca being the most effective.
当用1,4 - 丁二胺处理含有溶血补体第一（C′）、第四（C′）和第二（C′）成分的致敏红细胞（EA）时，C′从细胞中被去除，而C′和C′未被去除。所有两端带有氨基的3至8个碳的直链二胺去除C′的能力大致相同；二胺的活性比相应的单胺高得多。C′从红细胞中被二胺去除后可在溶液中回收。在最初的10分钟内，C′的脱离速率相当高，之后变慢并遵循一级动力学。似乎C′和二胺在致敏红细胞上竞争相同的位点。以下观察结果表明Ca在EAC′键中起作用：（）Ca浓度增加会减弱二胺的作用。（）EAC′键在低二价阳离子浓度下解离。（）一些二价阳离子能维持EAC′键，其中Ca最有效。
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