ORLANS E, ROSE M E
Immunology. 1965 Feb;8(2):193-205.
The increased precipitation of fowl antibody with antigen in the presence of normal fresh fowl serum (NFS) is due to one or more normal serum factors, including a macroglobulin, which resist heating at 56° for 30 minutes, but are inhibited by EDTA and destroyed at pH values below 5.5. Dialysis against low ionic strength buffers at neutral pH values concentrates the factors in the euglobulin fraction, and with antisera yields antibody-containing pseudoglobulin preparations free of macroglobulin. Enhanced precipitation occurs both in 0.9 and 8 per cent NaCl, but the effect is greater at the lower salt concentration. Its magnitude depends on the antibody preparation, the antigen/antibody ratio and on the concentration (rather than the amount) of the normal serum factors. Its relation to haemolytic complement has been studied but remains obscure.
在正常新鲜禽血清（NFS）存在的情况下，禽类抗体与抗原沉淀增加是由于一种或多种正常血清因子，包括一种巨球蛋白，其能抵抗56℃加热30分钟，但被EDTA抑制并在pH值低于5.5时被破坏。在中性pH值下用低离子强度缓冲液透析可使这些因子在优球蛋白部分浓缩，并且与抗血清一起可产生不含巨球蛋白的含抗体假球蛋白制剂。在0.9%和8%的NaCl中均会出现沉淀增强，但在较低盐浓度下效果更明显。其程度取决于抗体制剂、抗原/抗体比例以及正常血清因子的浓度（而非量）。已经对其与溶血补体的关系进行了研究，但仍不清楚。
