BRINSTER R L
J Exp Zool. 1965 Feb;158:59-68. doi: 10.1002/jez.1401580106.
The effect of various possible energy sources on the development of two-cell mouse ova into blastocysts was examined. Energy for development of two-cell mouse ova could be supplied by lactate, pyruvate, oxaloacetate, or phosphoenolpyruvate. Compounds such as glucose, fructose, ribose, glucose-6-phosphate, fructose-1, 6-phosphate, acetate, citrate, α-ketoglutarate, succinate, fumarate, and malate could not provide energy for development of two-cell mouse ova. The optimum concentrations at pH 7.38 for those compounds which would supply energy was 5.00 × 10 lactate, 3.16 × 10 pyruvate, 3.16 × 10 oxaloacetate, and 1.00 × 10 phosphoenolpyruvate. The possibility that interactions existed between the effects of osmolarity, pH, and energy source was examined in several experiments. There was no interaction between the effects of osmolarity and pH or osmolarity and the four possible energy sources. However, there was a significant interaction between energy source and pH. The result of this is that an increase in pH of the medium results in an increase in the optimum concentration of the compound supplying energy to the developing ova.
研究了各种可能的能量来源对双细胞小鼠卵发育成胚泡的影响。双细胞小鼠卵发育所需的能量可由乳酸、丙酮酸、草酰乙酸或磷酸烯醇丙酮酸提供。葡萄糖、果糖、核糖、6-磷酸葡萄糖、1,6-二磷酸果糖、乙酸盐、柠檬酸盐、α-酮戊二酸、琥珀酸盐、富马酸盐和苹果酸盐等化合物不能为双细胞小鼠卵的发育提供能量。在pH 7.38时，那些能提供能量的化合物的最佳浓度分别为5.00×10乳酸、3.16×10丙酮酸、3.16×10草酰乙酸和1.00×10磷酸烯醇丙酮酸。在几个实验中研究了渗透压、pH和能量来源的影响之间是否存在相互作用。渗透压和pH的影响之间或渗透压与四种可能的能量来源之间不存在相互作用。然而，能量来源和pH之间存在显著的相互作用。其结果是，培养基pH的升高导致向发育中的卵提供能量的化合物的最佳浓度增加。
