Pettersson K J, Nilsson L B
Department of Bioanalysis, Astra Arcus AB, Södertälje, Sweden.
J Chromatogr. 1992 Oct 2;581(1):161-4. doi: 10.1016/0378-4347(92)80462-y.
A simple and sensitive procedure for the routine assay of the analgesic drug dextropropoxyphene and its main metabolite, norpropoxyphene, in plasma is described. After liquid-liquid extraction from alkalinized plasma and back-extraction into a small volume of an acidic aqueous phase, the aqueous phase was injected into a column packed with 3-microns octadecylsilica particles. Ultraviolet absorbance detection at 210 nm was used. Concentrations down to 2 nM could be determined for both compounds; at this level, the intra-assay coefficient of variation was 5%.
