血红蛋白和珠蛋白链的毛细管电泳

Capillary electrophoresis of hemoglobins and globin chains.

作者信息

Zhu M, Rodriguez R, Wehr T, Siebert C

机构信息

Analytical Systems Division, Bio-Rad Laboratories, Hercules, CA 94547.

出版信息

J Chromatogr. 1992 Sep 11;608(1-2):225-37. doi: 10.1016/0021-9673(92)87128-u.

DOI:10.1016/0021-9673(92)87128-u

PMID:1430026

Abstract

Capillary isoelectric focusing (cIEF) and free zone capillary electrophoresis were evaluated for separation of native hemoglobins and globin chains. High-resolution separations of adult human hemoglobin A, fetal human hemoglobin F, and hemoglobin variants S and C were obtained using cIEF with cathodic mobilization. Absorbance detection in the UV and visible regions were compared, and on-line fast UV or visible-wavelength scanning detection was used to obtain spectral information on separated components. Globin chain analysis was performed on the same hemoglobin species by free zone capillary electrophoresis following precipitation of the protein with acidic acetone. Free zone separations were carried out at low pH in the presence of 7 M urea.

摘要

对毛细管等电聚焦（cIEF）和自由区毛细管电泳进行了评估，以分离天然血红蛋白和珠蛋白链。使用带阴极迁移的cIEF实现了成人血红蛋白A、胎儿血红蛋白F以及血红蛋白变体S和C的高分辨率分离。比较了紫外和可见光区域的吸光度检测，并使用在线快速紫外或可见波长扫描检测来获取分离组分的光谱信息。在蛋白质用酸性丙酮沉淀后，通过自由区毛细管电泳对相同的血红蛋白种类进行珠蛋白链分析。自由区分离在低pH值且存在7 M尿素的条件下进行。