Francina A, Baudonnet C, Dorléac E, Galacteros F, Delaunay J
Hemoglobin. 1982;6(5):481-91. doi: 10.3109/03630268209083761.
A simple isoelectric focusing technique is presented for the screening of alpha- and beta-globin chain variants on polyacrylamide gel in the presence of 8 M urea. No detergent was used. Variants focused at discrete pH values, depending on the charge variation (-2, -1, 0, +1 or +2) introduced by the mutation. A linear relationship was evidenced between the charge variation and the shift of the apparent isoelectric point of the variants. The practical interest of this procedure is illustrated.
本文介绍了一种简单的等电聚焦技术，用于在8M尿素存在下，在聚丙烯酰胺凝胶上筛选α和β珠蛋白链变体。未使用去污剂。变体在离散的pH值处聚焦，这取决于突变引入的电荷变化（-2、-1、0、+1或+2）。变体的电荷变化与表观等电点的偏移之间呈现出线性关系。文中阐述了该方法的实际应用价值。
