Chaudhuri P P, Sengupta K, Manna B, Saha M K, Pal S C, Das P
Department of Parasitology, National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
J Diarrhoeal Dis Res. 1992 Sep;10(3):151-5.
The roles of circulating anti-Giardia IgM and IgG antibodies were assayed by the Enzyme linked immunosorbent assay (ELISA) using axenic Giardia lamblia whole trophozoites as antigen for routine serodiagnosis of Giardia infection. Detection of anti-Giardia IgM antibody was found to be useful in the early detection of symptomatic giardiasis, whereas IgG detection was not, since the latter could not discriminate between present infection and previous exposure. A mean optical density (OD) +/- 2 standard deviation (SD) value of 0.088 at 1:100 serum dilution was found optimal for the detection of anti-Giardia IgM antibodies as it completely separated out the acute Giardia cases from the Giardia-free controls. The same sera did not reveal any marked differences when tested for anti-Giardia IgG antibodies. Detection of IgM antibodies correlated with high sensitivity and specificity (96%) in an evaluation of a large number of sera collected from Giardia cases and controls.
采用酶联免疫吸附测定法（ELISA），以无菌蓝氏贾第鞭毛虫全滋养体作为抗原，检测循环抗贾第鞭毛虫IgM和IgG抗体，用于贾第鞭毛虫感染的常规血清学诊断。结果发现，抗贾第鞭毛虫IgM抗体检测有助于有症状贾第鞭毛虫病的早期诊断，而IgG检测则不然，因为后者无法区分当前感染和既往接触情况。在血清稀释度为1:100时，平均光密度（OD）±2标准差（SD）值为0.088被认为是检测抗贾第鞭毛虫IgM抗体的最佳值，因为它能将急性贾第鞭毛虫病例与无贾第鞭毛虫对照完全区分开来。检测相同血清中的抗贾第鞭毛虫IgG抗体时，未发现任何显著差异。在对从贾第鞭毛虫病例和对照中收集的大量血清进行评估时，IgM抗体检测具有高敏感性和特异性（96%）。
