Winiecka J, Kasprzak W, Kociecka W, Płotkowiak J, Myjak P
Tropenmed Parasitol. 1984 Mar;35(1):20-2.
Anti-Giardia antibodies were studied in 88 sera of patients with symptomatic and asymptomatic giardiasis by using the indirect immunofluorescent test and Giardia intestinalis trophozoites from an axenic culture as antigen. The sera of 40 healthy subjects were used as a control group and of 28 patients with other intestinal parasites (14 with Sarcocystis sp. and 14 with symptomatic Entamoeba histolytica infection) have been investigated for determination of test specificity. 82% of sera from patients with giardiasis presented G. intestinalis antibodies at titers of 1:16 to 1:256. Titers of at least 1:32, accepted as diagnostic and indicative of the infection, were found in 66% of patients with giardiasis. All of 18 patients with acute course of giardiasis had titers of at least 1:32. Only 5 sera (7%) from healthy control subjects or from patients infected with other intestinal parasites reacted at dilutions of 1: greater than or equal to 32. The indirect immunofluorescence test proves to be useful in detecting antibodies to G. intestinalis in the serum. However, the negative results occurring rather frequently in persons with chronic infection indicate a need for a more critical evaluation of serological tests in giardiasis.
采用间接免疫荧光试验，以无菌培养的肠贾第虫滋养体为抗原，对88例有症状和无症状贾第虫病患者的血清进行了抗贾第虫抗体研究。40名健康受试者的血清用作对照组，并对28例患有其他肠道寄生虫的患者（14例感染肉孢子虫属，14例有症状的溶组织内阿米巴感染）进行了检测，以确定试验的特异性。82%的贾第虫病患者血清中出现了效价为1:16至1:256的肠贾第虫抗体。在66%的贾第虫病患者中发现了至少1:32的效价，该效价被视为诊断感染的依据并表明存在感染。18例急性贾第虫病患者的效价均至少为1:32。健康对照受试者或感染其他肠道寄生虫患者的血清中只有5份（7%）在稀释度为1:大于或等于32时出现反应。间接免疫荧光试验被证明可用于检测血清中抗肠贾第虫抗体。然而，慢性感染患者中经常出现阴性结果，这表明需要对贾第虫病的血清学检测进行更严格的评估。
