从专性嗜盐细菌中纯化一种需盐酶
PURIFICATION OF A SALT-REQUIRING ENZYME FROM AN OBLIGATELY HALOPHILIC BACTERIUM.
作者信息
HOLMES P K, HALVORSON H O
出版信息
J Bacteriol. 1965 Aug;90(2):312-5. doi: 10.1128/jb.90.2.312-315.1965.
Abstract
Holmes, P. K. (University of Illinois, Urbana), and H. O. Halvorson. Purification of a salt-requiring enzyme from an obligately halophilic bacterium. J. Bacteriol. 90: 312-315. 1965.-The discovery that some halophilic enzymes may be reactivated from the salt-free and inactive state has facilitated the purification of nicotinamide adenine dinucleotide-linked malic acid dehydrogenase from crude extracts of Halobacterium salinarium. In the absence of large amounts of salt, the enzyme was totally inactive; yet, in this salt-free state, it could be purified by conventional techniques. The highly purified halophilic enzyme was shown to require a high concentration of salt for activity.
摘要
福尔摩斯，P.K.（伊利诺伊大学厄巴纳分校）和H.O.哈尔沃森。从专性嗜盐细菌中纯化一种需盐酶。《细菌学杂志》90: 312 - 315。1965年。——发现一些嗜盐酶可能从无盐且无活性的状态重新激活，这有助于从盐生盐杆菌的粗提取物中纯化烟酰胺腺嘌呤二核苷酸连接的苹果酸脱氢酶。在没有大量盐的情况下，该酶完全无活性；然而，在这种无盐状态下，它可以通过传统技术进行纯化。高度纯化的嗜盐酶被证明需要高浓度的盐来激活活性。
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