Jones J V, James H, Tan M H, Mansour M
Department of Medicine, Dalhousie University, Halifax, NS, Canada.
J Rheumatol. 1992 Sep;19(9):1397-402.
IgG was isolated from 9 patients with high levels of anticardiolipin antibodies (aCL). The standard ELISA was modified, using gelatin to postcoat ELISA plates and as diluent. Under these conditions, 8/9 samples of IgG bound to cardiolipin only in the presence of a cofactor, found in fetal calf serum and normal human serum (NHS). beta 2-glycoprotein I (apolipoprotein H) was at least as effective as NHS as a cofactor for 7/9 IgG samples. Our studies confirm that for some aCL detected in conventional assays, the antibody will react with cardiolipin only if beta 2-glycoprotein I is present.
从9名抗心磷脂抗体（aCL）水平较高的患者中分离出IgG。对标准酶联免疫吸附测定（ELISA）进行了改良，使用明胶包被ELISA板并作为稀释剂。在这些条件下，9份IgG样本中有8份仅在存在于胎牛血清和正常人血清（NHS）中的辅因子存在时才与心磷脂结合。β2-糖蛋白I（载脂蛋白H）作为7/9 IgG样本的辅因子，其效果至少与NHS一样好。我们的研究证实，对于传统检测中检测到的某些aCL，只有在存在β2-糖蛋白I时，抗体才会与心磷脂发生反应。
