Miller S C, Jee W S
Calcif Tissue Res. 1977 Oct 20;23(3):207-14. doi: 10.1007/BF02012787.
Male rats weighing 100 g were assigned to groups and injected daily for 10 days with vehicle (control), 0.4, 2.0, 4.0, 10.0, or 20.0 mg/kg/day of ethane-1-hydroxy-1,1-diphosphonate (EHDP) or dichloromethylene diphosphonate (C12MDP). The proximal tibial metaphysis and epiphysis were assayed for changes in percentage of hard tissue and bone formation parameters. From the data, information about hard tissue resorption was deduced. All doses of C12MDP and doses of 2.0 mg EHDP/kg/day and greater caused significant increases in percentage of hard tissues with C12MDP being more effective than similar doses of EHDP in decreasing bone resorption. Osteoclast population parameters were increased with all doses of both C12MDP and EHDP with C12MDP having a greater effect than similar doses of EHDP. Decreases in the proliferation of the osteoprogenitor pool parallel the decreases in osteoblasts and bone formation parameters. These decreases in osteoprogenitor pool proliferation do not account for the increases with diphosphonates in osteoclast population parameters.
将体重100克的雄性大鼠分组，连续10天每天注射赋形剂（对照组）、0.4、2.0、4.0、10.0或20.0毫克/千克/天的乙烷-1-羟基-1,1-二膦酸盐（EHDP）或二氯亚甲基二膦酸盐（C12MDP）。检测胫骨近端干骺端和骨骺的硬组织百分比变化以及骨形成参数。根据这些数据推断出有关硬组织吸收的信息。所有剂量的C12MDP以及2.0毫克EHDP/千克/天及以上剂量均导致硬组织百分比显著增加，C12MDP在减少骨吸收方面比类似剂量的EHDP更有效。两种药物所有剂量均使破骨细胞群体参数增加，且C12MDP的作用大于类似剂量的EHDP。骨祖细胞池增殖的减少与成骨细胞和骨形成参数的减少平行。骨祖细胞池增殖的这些减少并不能解释双膦酸盐导致破骨细胞群体参数增加的现象。
