悬浮状态下分离的大鼠肝细胞的呼吸作用。
THE RESPIRATION OF ISOLATED RAT-HEPATIC CELLS IN SUSPENSION.
作者信息
IYPE P T, BHARGAVA P M
出版信息
Biochem J. 1965 Jan;94(1):284-8. doi: 10.1042/bj0940284.
Abstract
- Rat-hepatic cells in suspension have been shown to have an endogenous respiration of 5.6+/-0.17 when suspended in 0.1 m-sucrose and 0.02 m-tris-hydrochloric acid buffer. The respiration in 0.25 m-sucrose and 0.02 m-tris-hydrochloric acid buffer is 30-40% less. 2. Potassium chloride (0.05 m) is slightly inhibitory and calcium chloride (0.0025 m) highly inhibitory to endogenous respiration of the hepatic cells in suspension. The cells do not respire in Krebs-Ringer phosphate buffer. 3. The respiration of the hepatic cells in suspension is stimulated by pyruvate, citrate, isocitrate, oxoglutarate, succinate, fumarate, malate and glutamate; there is no significant stimulation (or inhibition) by glucose, fructose, acetate and butyrate. In almost all the cases where stimulation was observed, it was found that the higher the endogenous respiration the lower is the stimulation.
摘要
- 悬浮于0.1m蔗糖和0.02m三羟甲基氨基甲烷盐酸缓冲液中的大鼠肝细胞，其内源呼吸为5.6±0.17。悬浮于0.25m蔗糖和0.02m三羟甲基氨基甲烷盐酸缓冲液中的呼吸则减少30 - 40%。2. 0.05m氯化钾对悬浮肝细胞的内源呼吸有轻微抑制作用，0.0025m氯化钙有高度抑制作用。细胞在磷酸缓冲液中不进行呼吸。3. 悬浮肝细胞的呼吸受到丙酮酸、柠檬酸、异柠檬酸、酮戊二酸、琥珀酸、延胡索酸、苹果酸和谷氨酸的刺激；葡萄糖、果糖、乙酸和丁酸无明显刺激（或抑制）作用。在几乎所有观察到刺激作用的情况下，发现内源呼吸越高，刺激作用越低。
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