从大鼠肝脏中酶法制备分离的完整实质细胞。
The enzymatic preparation of isolated intact parenchymal cells from rat liver.
作者信息
Howard R B, Christensen A K, Gibbs F A, Pesch L A
出版信息
J Cell Biol. 1967 Dec;35(3):675-84. doi: 10.1083/jcb.35.3.675.
Abstract
Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light and electron microscopy, approximately 75% of the isolated cells retain their structural integrity. The cell suspensions are capable of maintaining endogenous respiration in the presence of 1% albumin for periods of time up to 8 hr. These cell preparations consist almost entirely of parenchymal cells and offer a unique tissue preparation for the study of hepatic metabolism.
摘要
通过用胶原酶和透明质酸酶孵育，随后进行机械处理，从大鼠肝脏制备分离的实质细胞悬液。使用0.15%的胶原酶与0.15%的透明质酸酶一起可产生足够数量的细胞用于实验目的。如光学显微镜和电子显微镜所示，约75%的分离细胞保持其结构完整性。细胞悬液在1%白蛋白存在下能够维持内源性呼吸长达8小时。这些细胞制剂几乎完全由实质细胞组成，为研究肝脏代谢提供了一种独特的组织制剂。
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本文引用的文献
1
Relation of oxygen and temperature in the preservation of tissues by refrigeration.冷藏保存组织中氧气与温度的关系。
Proc Soc Exp Biol Med. 1949 Jun;71(2):196-200. doi: 10.3181/00379727-71-17131.
2
THE RESPIRATION OF ISOLATED RAT-HEPATIC CELLS IN SUSPENSION.悬浮状态下分离的大鼠肝细胞的呼吸作用。
Biochem J. 1965 Jan;94(1):284-8. doi: 10.1042/bj0940284.
3
RESPIRATORY CONTROL OF DISPERSED RAT-LIVER CELLS.分散的大鼠肝细胞的呼吸控制
Biochim Biophys Acta. 1965 Jan 4;97:1-8. doi: 10.1016/0304-4165(65)90262-x.
4
5
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
J Biol Chem. 1964 Feb;239:375-80.
6
[STUDY OF THE METABOLIC BEHAVIOR OF ISOLATED LIVER CELLS].[离体肝细胞代谢行为的研究]
Sperimentale. 1963 Sep-Oct;113:251-8.
7
DISASSOCIATION OF HEART CELLS BY COLLAGENASE.用胶原酶分离心肌细胞
Nature. 1963 Oct 19;200:261-2. doi: 10.1038/200261b0.
8
[FACTORS WHICH CONDITION THE ULTRASTRUCTURAL INTEGRITY OF ISOLATED HEPATIC CELLS].[影响离体肝细胞超微结构完整性的因素]
Sperimentale. 1963 May-Jun;113:119-24.
9
A new method for the preparation of liver cell suspensions.一种制备肝细胞悬液的新方法。
Exp Cell Res. 1962 Sep;27:453-67. doi: 10.1016/0014-4827(62)90011-3.
10
Isolation of intact liver cells.完整肝细胞的分离
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