Lubowitz H
J Pharmacol Exp Ther. 1977 Oct;203(1):92-6.
Bumetanide, a sulfamyl-aminobenzoic acid derivative, is a new and highly effective diuretic agent. The present studies were designed to examine its effects on cation transport in human red cells. At a concentration of 10(-3) M, the drug inhibited both active and passive unidirectional sodium fluxes, as well as active potassium influx. It also caused a significant inhibition of glycolysis. The inhibition caused by bumetanide was less than that seen with ouabain alone, but a bumetanide effect was also present in ouabain-treated cells. Bumetanide had no effect on red cell Na-K adenosine triphosphatase activity and did not affect net transport of sodium in sodium-loaded cells. The data are consistent with a model in which the inhibition of monovalent cation movement in red cells by bumetanide is related to an effect of this compound in decreasing the permeability of the red cell membrane to sodium.
布美他尼是一种氨磺酰氨基苯甲酸衍生物，是一种新型高效利尿剂。本研究旨在考察其对人红细胞阳离子转运的影响。在浓度为10⁻³M时，该药物抑制主动和被动单向钠通量以及主动钾内流。它还显著抑制糖酵解。布美他尼引起的抑制作用小于单独使用哇巴因时的抑制作用，但在哇巴因处理的细胞中也存在布美他尼效应。布美他尼对红细胞钠钾三磷酸腺苷酶活性无影响，也不影响钠负荷细胞中钠的净转运。这些数据与一个模型一致，即布美他尼对红细胞单价阳离子运动的抑制与该化合物降低红细胞膜对钠的通透性的作用有关。
