Klobusická M, Kusenda J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Neoplasma. 1992;39(5):291-7.
Enzyme characteristics of in vitro activated peritoneal macrophages of normal and BP6-TU2 tumor-bearing rats were compared with those of nonactivated macrophages. The activating effect of LPS, IFN-gamma, IL-2, TPA, TNF and Zymosan was assessed by the determination of the activities of alpha-naphthyl butyrate esterase (ANBE), tartrate-resistant acid phosphatase (TRAP), beta glucuronidase (BG) and 5'nucleotidase (5'NT). The used individual activators induced the macrophage enzyme activities in different degrees. LPS, TPA and TNF appeared to be the most effective activators of enzyme activities in macrophages of normal rats. IFN-gamma, IL-2 and Zymosan were less effective. The macrophages of BP6-TU2 tumor-bearing rats were less sensitive to the stimulatory effect of activators with respect to their enzyme activation capacity, except for TRAP activity. The results indicate that enzyme ANBE is an adequate marker of rat peritoneal macrophages. Enzyme TRAP determines the macrophage activation degree more expressively, in comparison with BG and 5'NT. The differences in enzyme activities could be a suitable marker of macrophage activation and might suggest the dependence on the pathway of macrophage stimulation by distinct activators.
将正常大鼠和携带BP6-TU2肿瘤大鼠体外活化的腹腔巨噬细胞的酶特性与未活化的巨噬细胞进行了比较。通过测定α-萘丁酸酯酶（ANBE）、抗酒石酸酸性磷酸酶（TRAP）、β-葡萄糖醛酸酶（BG）和5'-核苷酸酶（5'NT）的活性，评估了脂多糖（LPS）、γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）、佛波酯（TPA）、肿瘤坏死因子（TNF）和酵母聚糖的激活作用。所使用的各个激活剂均不同程度地诱导了巨噬细胞的酶活性。LPS、TPA和TNF似乎是正常大鼠巨噬细胞中酶活性最有效的激活剂。IFN-γ、IL-2和酵母聚糖的效果较差。除TRAP活性外，携带BP6-TU2肿瘤大鼠的巨噬细胞对激活剂刺激作用的酶激活能力较不敏感。结果表明，酶ANBE是大鼠腹腔巨噬细胞的合适标志物。与BG和5'NT相比，酶TRAP更能明显地确定巨噬细胞的激活程度。酶活性的差异可能是巨噬细胞激活的合适标志物，并可能提示不同激活剂对巨噬细胞刺激途径的依赖性。
