Kalafut F, Kusenda J, Klobusická M, Novotná L
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Neoplasma. 1990;37(4):405-14.
The peritoneal macrophages from normal Lewis rats were characterized by their capacity to phagocytose, by the presence of nonspecific esterase and Fc receptors. In vitro, these macrophages were maintained in culture 7 and/or 21 days, respectively, and for the last 24 h (activation period) were cultured with 0.1-4.0 micrograms/ml of lipopolysaccharide (LPS) and were found tumoricidal against different rat fibrosarcomas, at a ratio of 50:1 (BP6-Tu2, MC-1 and B77). Macrophage-mediated tumor cytolysis was determined using 51Cr-release assay. The sensitivity of used tumors to macrophage-mediated cyto-toxicity was different. In vivo the transfer of the activated macrophages together with the mentioned tumor cells to rats inhibited the growth of tumors. The adoptive transfer of macrophages activated with "activators" might lead to a new kind of immunotherapy of neoplastic diseases.
正常Lewis大鼠的腹膜巨噬细胞具有吞噬能力、非特异性酯酶和Fc受体。在体外，这些巨噬细胞分别培养7天和/或21天，在最后24小时（激活期）用0.1 - 4.0微克/毫升的脂多糖（LPS）培养，发现它们对不同的大鼠纤维肉瘤具有杀瘤作用，比例为50:1（BP6 - Tu2、MC - 1和B77）。使用51Cr释放试验测定巨噬细胞介导的肿瘤细胞溶解作用。所用肿瘤对巨噬细胞介导的细胞毒性的敏感性不同。在体内，将活化的巨噬细胞与上述肿瘤细胞一起转移给大鼠可抑制肿瘤生长。用“激活剂”激活的巨噬细胞的过继转移可能会导致一种新型的肿瘤疾病免疫疗法。
