Swain M G, Heyes M P, Vergalla J, Jones E A
Liver Diseases Section, NIDDK, National Institutes of Health, Bethesda, MD 20892.
Neurosci Lett. 1992 Jul 20;141(2):243-6. doi: 10.1016/0304-3940(92)90904-l.
In order to determine whether acute toxic hepatitis in the rat is associated with an accumulation of methionine enkephalin in plasma and increased blood-to-brain transfer of methionine enkephalin, immunoreactive methionine enkephalin levels were determined by radioimmunoassay in plasma, cerebrospinal fluid and whole brain samples from rats with thioacetamide induced acute toxic hepatitis. Thioacetamide treatment was associated with an 8.7-fold increase in plasma immunoreactive methionine enkephalin levels (P less than or equal to 0.005) 24 h after treatment. However, this marked elevation in plasma immunoreactive methionine enkephalin levels was not associated with an increase in whole brain or cerebrospinal fluid immunoreactive methionine enkephalin levels. These data suggest that increased plasma-to-brain transfer of methionine enkephalin does not occur in this model of acute toxic hepatitis.
为了确定大鼠急性中毒性肝炎是否与血浆中甲硫氨酸脑啡肽的蓄积以及甲硫氨酸脑啡肽血脑转运增加有关，采用放射免疫分析法测定了硫代乙酰胺诱导的急性中毒性肝炎大鼠的血浆、脑脊液和全脑样本中的免疫反应性甲硫氨酸脑啡肽水平。硫代乙酰胺治疗后24小时，血浆免疫反应性甲硫氨酸脑啡肽水平增加了8.7倍（P≤0.005）。然而，血浆免疫反应性甲硫氨酸脑啡肽水平的这种显著升高与全脑或脑脊液免疫反应性甲硫氨酸脑啡肽水平的增加无关。这些数据表明，在这种急性中毒性肝炎模型中，甲硫氨酸脑啡肽的血浆到脑的转运没有增加。
