HARFORD C G, HAMLIN A, PARKER E
J Exp Med. 1955 Jun 1;101(6):577-90. doi: 10.1084/jem.101.6.577.
Recently improved methods for visualization of thin tissue sections by electron microscopy have been applied to the study of early changes in the bronchial epithelium of mice infected by inhalation of aerosols of influenza virus. In confirmation of previous findings by the authors, inclusion bodies have been demonstrated in ciliated and non-ciliated cells of infected bronchial epithelium. In addition to 3 strains of mouse-adapted Type A virus, 2 unadapted strains gave qualitatively the same results. The inclusion bodies were found to be composed largely of particles of a size estimated to correspond to the known size of influenza virus. The viral lesion of the cytoplasm was also associated with linear formations which were thought to be abnormal forms of endoplasmic reticulum. Well developed microvilli were found on the ciliated borders of ciliated cells, but no evidence was found of viral growth in this region.
最近，通过电子显微镜观察薄组织切片的方法有所改进，并已应用于研究吸入流感病毒气溶胶感染的小鼠支气管上皮的早期变化。为证实作者先前的发现，在受感染支气管上皮的纤毛细胞和非纤毛细胞中均发现了包涵体。除3株适应小鼠的甲型病毒外，2株未适应的病毒也给出了定性相同的结果。发现包涵体主要由大小估计与已知流感病毒大小相对应的颗粒组成。细胞质的病毒损伤还与线性结构有关，这些线性结构被认为是内质网的异常形式。在纤毛细胞的纤毛边界发现了发育良好的微绒毛，但未发现该区域有病毒生长的证据。
