LASFARGUES E Y, MOORE D H, MURRAY M R, HAAGENSEN C D, POLLARD E C
J Biophys Biochem Cytol. 1959 Jan 25;5(1):93-6. doi: 10.1083/jcb.5.1.93.
Thin sections of tissue cultures grown from tumors of the RIII high-breast-cancer strain mice were studied in the electron microscope. These tissues contain an abundance of particles whose morphology is consistent with biophysical measurement of the milk agent. These particles, found only extracellularly in our cultures, are formed at the cell membrane. The process of formation, as reconstructed from sections, appears to include a thickening and protrusion of the cell membrane which then evolves gradually into a dense sphere and separates from the cell in much the same manner as does influenza virus. The contents of the newly formed body are later rearranged to form a nucleoid within a membranous sac.
对从RIII高乳腺癌品系小鼠肿瘤中培养出的组织薄片进行了电子显微镜研究。这些组织含有大量颗粒，其形态与乳因子的生物物理测量结果一致。这些颗粒仅在我们培养物的细胞外被发现，是在细胞膜处形成的。从切片重建的形成过程似乎包括细胞膜增厚和突出，然后逐渐演变成一个致密球体，并以与流感病毒非常相似的方式与细胞分离。新形成物体的内容物随后重新排列，在膜囊中形成类核。
