Andersson A, Buschard K
Trans Am Soc Artif Intern Organs. 1977;23:342-6.
The aim of this study was to assess the curative effects of long-term stored islets, when transplanted into diabetic recipients. For this purpose mouse pancreatic islets, either freshly isolated or cultured for 10 days, were allografted intraperitoneally into noninbred mice or into nude mice, all made diabetic by means of a streptozotocin injection. When implanted into nude mice, which lack a cell-mediated immune response, cultured islets were equally effective as were freshly isolated islets, in reducing the hyperglycemia. When fresh or cultured islets were allografted into diabetic NMRI mice there was only a short and transient reduction of the blood sugar, suggesting that no reduction of the antigenicity of the islets had occurred after 10 days of culture. The culture system, however, may serve as a purification step, after which only functionally competent islets remain.
本研究的目的是评估长期保存的胰岛移植到糖尿病受体后产生的疗效。为此，将新鲜分离的或培养10天的小鼠胰岛腹腔内移植到通过链脲佐菌素注射诱导糖尿病的非近交系小鼠或裸鼠体内。当移植到缺乏细胞介导免疫反应的裸鼠体内时，培养的胰岛在降低高血糖方面与新鲜分离的胰岛同样有效。当将新鲜的或培养的胰岛同种异体移植到糖尿病NMRI小鼠体内时，血糖仅出现短暂下降，这表明培养10天后胰岛的抗原性并未降低。然而，培养系统可作为一个纯化步骤，在此之后仅保留功能正常的胰岛。
