Rijkelijkhuizen Josephine K R A, van der Burg Michael P M, Töns Annemiek, Terpstra Onno T, Bouwman Eelco
Department of Surgery, Leiden University Medical Centre, Leiden, The Netherlands.
Pancreas. 2006 May;32(4):403-7. doi: 10.1097/01.mpa.0000220866.87658.b2.
The pig is generally considered a suitable alternative donor for clinical islet transplantation. However, adult pig islets are difficult to isolate and culture, often behave variably in in vitro assays, and do not consistently cure diabetic nude mice. In this study, we compared the in vivo function of freshly isolated and cultured adult porcine islets by transplantation in diabetic nude mice.
Freshly isolated and cultured islets were transplanted in different doses to diabetic nude mice (N = 48).
Average islet yield was 1924 islet-equivalents per gram of pancreas, purity 96%, and the viability that was measured by acridine orange and propidium iodide was greater than 80% in all freshly isolated islet preparations. Grafts of freshly isolated islets failed to reduce hyperglycemia in 17 of 18 recipients. Although after 1 day of culture islet recovery was only 21%, grafts of these islets cured 12 of 17 mice. After 7 to 14 days of culture, the recovery had decreased to 11%; however, these islets reversed hyperglycemia in all mice (13/13) and showed shorter time-to-normoglycemia and more tightly regulated blood glucose.
Although freshly isolated adult porcine islets survive culture and transplantation poorly, islets selected by prolonged culture are of high potential.
猪通常被认为是临床胰岛移植合适的替代供体。然而，成年猪胰岛难以分离和培养，在体外试验中常常表现各异，并且不能始终治愈糖尿病裸鼠。在本研究中，我们通过将新鲜分离和培养的成年猪胰岛移植到糖尿病裸鼠体内，比较了它们的体内功能。
将新鲜分离和培养的胰岛以不同剂量移植到糖尿病裸鼠（N = 48）体内。
平均胰岛产量为每克胰腺1924个胰岛当量，纯度为96%，所有新鲜分离的胰岛制剂中，用吖啶橙和碘化丙啶测定的活力均大于80%。新鲜分离的胰岛移植未能使18只受体中的17只降低高血糖。虽然培养1天后胰岛回收率仅为21%，但这些胰岛移植治愈了17只小鼠中的12只。培养7至14天后，回收率降至11%；然而，这些胰岛使所有小鼠（13/13）的高血糖得到逆转，并且达到正常血糖的时间更短，血糖调节更严格。
虽然新鲜分离的成年猪胰岛在培养和移植后的存活情况不佳，但经过长时间培养筛选的胰岛具有很高的潜力。
