Edwin S S, Mitchell M D
Department of Obstetrics and Gynecology, University of Utah, School of Medicine, Salt Lake City 84132.
Prostaglandins Leukot Essent Fatty Acids. 1992 Sep;47(1):77-81. doi: 10.1016/0952-3278(92)90189-p.
We have evaluated the production of prostaglandin E2 (PGE2) and its regulation in amnion, chorion, and decidual cells in the presence and absence of fetal calf serum (FCS), and in the absence of FCS but with supplementation with substrate arachidonic acid (AA). Basal rates of PGE2 biosynthesis in amnion, chorion and decidual cell cultures were significantly reduced in the absence of FCS. The magnitudes of the responses to various stimulatory agents were different between cells from different tissues and the different culture media. We conclude that these different experimental conditions must be taken into account when interpreting the results of such in vitro experiments.
我们评估了在存在和不存在胎牛血清（FCS）的情况下，以及在不存在FCS但补充底物花生四烯酸（AA）的情况下，羊膜、绒毛膜和蜕膜细胞中前列腺素E2（PGE2）的产生及其调节。在不存在FCS的情况下，羊膜、绒毛膜和蜕膜细胞培养物中PGE2生物合成的基础速率显著降低。来自不同组织的细胞和不同培养基对各种刺激剂的反应程度不同。我们得出结论，在解释此类体外实验结果时，必须考虑这些不同的实验条件。
