Mitchell M D, LaMarche S, Adamson S, Coulam C, Silver R M, Edwin S S
Department of Obstetrics & Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Prostaglandins Leukot Essent Fatty Acids. 1994 Mar;50(3):137-40. doi: 10.1016/0952-3278(94)90096-5.
Activation of protein kinase C with phorbol esters stimulates prostaglandin (PG) biosynthesis in many cell types whereas down-regulation of protein kinase C can suppress stimulatory responses. Interleukin-1 beta (IL-1 beta) can stimulate PG production by intrauterine tissues and may play a significant part in the mechanisms of preterm labor associated with intrauterine infection. Hence we have evaluated the effects of staurosporine and H7 (inhibitors of protein kinase C) on IL-1 beta stimulation of amnion, chorion and decidual prostaglandin E2 (PGE2) production. Staurosporine and H7 alone were without effect on PGE2 production by any cell type. However with minor exceptions both protein kinase C inhibitors enhanced the stimulatory actions of IL-1 beta on PGE2 production by all three cell types. Hence we believe that protein kinase C is closely linked to the regulation of intrauterine PG biosynthesis and that these links may have multiple layers of complexity.
佛波酯激活蛋白激酶C可刺激多种细胞类型中前列腺素（PG）的生物合成，而蛋白激酶C的下调可抑制刺激反应。白细胞介素-1β（IL-1β）可刺激子宫内组织产生PG，并可能在与宫内感染相关的早产机制中起重要作用。因此，我们评估了星形孢菌素和H7（蛋白激酶C抑制剂）对IL-1β刺激羊膜、绒毛膜和蜕膜前列腺素E2（PGE2）产生的影响。单独使用星形孢菌素和H7对任何细胞类型的PGE2产生均无影响。然而，除了少数例外，两种蛋白激酶C抑制剂均增强了IL-1β对所有三种细胞类型PGE2产生的刺激作用。因此，我们认为蛋白激酶C与子宫内PG生物合成的调节密切相关，且这些联系可能具有多层复杂性。
