WHITE R G, COONS A H, CONNOLLY J M
J Exp Med. 1955 Jul 1;102(1):83-104. doi: 10.1084/jem.102.1.83.
After injection of ovalbumin as a water-in-oil emulsion a pronounced adjuvant effect is demonstrable following the incorporation of tubercle bacillary wax into the oily phase of the mixture. With single doses of antigen (10 mg. ovalbumin) there is a 4- to 5-fold increase in the amount of antibody at the median of 3 week serum levels in animals receiving a small dose of wax (40 microg.). With a 5 mg. dose of wax there is an 8-fold increase in serum antibody levels at the median. A striking feature of the action of wax is the stimulation of a macrophage proliferation locally at the site of injection, and the production of morphological abnormalities in these cells. As judged by staining techniques for antibody content, these locally assembled cells are not active in the formation of antibody. Wax injected in mineral oil results in a remarkable systematized stimulation of the reticulo-endothelial system. The greatly increased serum antibody levels demonstrated after the use of tubercle bacillary wax in antigen mixtures is attributed to a widespread proliferation of plasma cell elements in the lymphatic glands, spleen and liver.
将卵清蛋白作为油包水乳液注射后,在混合物的油相中加入结核杆菌蜡后,可显示出明显的佐剂效应。对于单剂量抗原(10毫克卵清蛋白),接受小剂量蜡(40微克)的动物在3周血清水平中位数时抗体量增加4至5倍。使用5毫克剂量的蜡时,血清抗体水平中位数增加8倍。蜡作用的一个显著特征是在注射部位局部刺激巨噬细胞增殖,并使这些细胞产生形态异常。通过抗体含量染色技术判断,这些局部聚集的细胞在抗体形成中不活跃。注入矿物油中的蜡会导致网状内皮系统受到显著的系统性刺激。在抗原混合物中使用结核杆菌蜡后血清抗体水平大幅升高,这归因于淋巴腺、脾脏和肝脏中浆细胞成分的广泛增殖。