Ho B C, Chew L M, Singh M, Yap E H
Department of Microbiology, Faculty of Medicine, National University of Singapore.
Southeast Asian J Trop Med Public Health. 1992 Jun;23(2):304-11.
The course of antibody production in Wistar neonatal and juvenile rats after primary infection with Breinlia booliati was studied by the DIG-ELISA technique using filter papers impregnated with capillary blood drawn from the infected rat tails at 7, 14, 28, 60 and 90 days post infection. Sera of neonatally infected rats did not react with adult worm antigen until day 7 and the titers of antibody remained at very low levels for the next 7 days. There was little tendency to eliminate the filarial larvae during this time. The antibody levels then rose rapidly throughout the next fortnight and increased to a maximum at day 60 after which the titer leveled out at a constant high value until early patency at day 90. On the other hand, antibodies could be detected in sera of juvenile infected rats as early as day 7 and the levels of antibody rose markedly to a maximum at day 28. During the period from day 60 to day 90 at early patency, the antibodies declined gradually to lower levels. The humoral immune responses of 42 neonatally infected rats and 53 juvenile infected rats of 3 strains (Lewis, Wistar and Sprague Dawley) were tested against soluble B. booliati antigens from both female (1:50) and male (1:10) worm extracts by the DIG-ELISA method. Antibodies were detected in sera from all the microfilaremic and amicrofilaremic rats belonging to neonatally and juvenile infected groups. Sera of clean neonatal rats did not give a positive reaction zone.
采用DIG-ELISA技术，通过在感染后7、14、28、60和90天采集感染大鼠尾巴的毛细血管血并浸渍于滤纸上，研究了Wistar新生和幼年大鼠初次感染布氏布鲁线虫后抗体产生的过程。新生感染大鼠的血清直到第7天才与成虫抗原发生反应，并且在接下来的7天里抗体滴度一直保持在很低的水平。在此期间几乎没有清除丝虫幼虫的趋势。随后在接下来的两周内抗体水平迅速上升，并在第60天达到最大值，此后滴度在恒定的高值保持平稳，直到第90天早期出现虫体。另一方面，幼年感染大鼠的血清早在第7天就能检测到抗体，并且抗体水平在第28天显著上升至最大值。在第60天至第90天早期出现虫体的期间，抗体逐渐下降至较低水平。通过DIG-ELISA方法，对3个品系（Lewis、Wistar和Sprague Dawley）的42只新生感染大鼠和53只幼年感染大鼠针对来自雌性（1:50）和雄性（1:10）虫体提取物的可溶性布氏布鲁线虫抗原的体液免疫反应进行了检测。在所有属于新生和幼年感染组的微丝蚴血症和无微丝蚴血症大鼠的血清中均检测到抗体。清洁新生大鼠的血清未出现阳性反应区。
