Glasier M A, Glutek S M, Hobkirk R
Department of Biochemistry, University of Western Ontario, London, Canada.
Steroids. 1992 Jun;57(6):295-300. doi: 10.1016/0039-128x(92)90063-f.
Guinea pig adrenal estrogen sulfotransferase from either sex was eluted as a single peak, irrespective of buffer salt concentration, when subjected to fast protein liquid chromatography on gel filtration columns. The same enzyme was consistently eluted in two distinct peaks during chromatofocusing. Adrenal pregnenolone sulfotransferase was eluted during gel filtration in a heterogeneous pattern, dependent on salt concentration. These properties have made possible almost complete separation of the two sulfotransferases in one step, although adrenal estrogen sulfotransferase may possess a minute intrinsic ability to catalyze sulfation of pregnenolone. Pregnenolone sulfotransferase had no measurable activity toward estrone. Pregnenolone sulfotransferase from both sexes yielded variable elution patterns during chromatofocusing. Estrogen sulfotransferase from the adrenal, as well as that of guinea pig chorion, was strongly inhibited by N-ethylmaleimide and to a lesser degree by iodoacetamide and iodoacetate. Adrenal and chorion estrogen sulfotransferases were thermolabile and were activated, although not protected from the effect of heat, by binding to 3'-phosphoadenosine 5'-phosphosulfate. Adrenal pregnenolone sulfotransferase was inhibited only by high concentrations of N-ethylmaleimide and not at all by iodoacetamide or iodoacetate. It was more thermostable than the estrogen sulfotransferase and was not activated by binding to 3'-phosphoadenosine 5'-phosphosulfate.
当在凝胶过滤柱上进行快速蛋白质液相色谱分析时，无论缓冲盐浓度如何，来自雌雄豚鼠的肾上腺雌激素磺基转移酶均以单一峰被洗脱。在色谱聚焦过程中，相同的酶始终以两个不同的峰被洗脱。肾上腺孕烯醇酮磺基转移酶在凝胶过滤过程中以异质模式被洗脱，这取决于盐浓度。这些特性使得在一步中几乎可以完全分离这两种磺基转移酶，尽管肾上腺雌激素磺基转移酶可能具有极微小的催化孕烯醇酮硫酸化的内在能力。孕烯醇酮磺基转移酶对雌酮没有可测量的活性。来自雌雄两性的孕烯醇酮磺基转移酶在色谱聚焦过程中产生可变的洗脱模式。肾上腺雌激素磺基转移酶以及豚鼠绒毛膜雌激素磺基转移酶受到N-乙基马来酰亚胺的强烈抑制，而受到碘乙酰胺和碘乙酸的抑制程度较小。肾上腺和绒毛膜雌激素磺基转移酶不耐热，并且通过与3'-磷酸腺苷5'-磷酸硫酸结合而被激活，尽管不能免受热的影响。肾上腺孕烯醇酮磺基转移酶仅受到高浓度N-乙基马来酰亚胺的抑制，而不受碘乙酰胺或碘乙酸的抑制。它比雌激素磺基转移酶更耐热，并且不通过与3'-磷酸腺苷5'-磷酸硫酸结合而被激活。
