Hobkirk R, Glasier M A
Department of Biochemistry, University of Western Ontario, London, Canada.
Biochem Cell Biol. 1992 Aug;70(8):712-5. doi: 10.1139/o92-108.
The highest total activity of estrogen sulfotransferase in guinea pig is in liver and the highest specific activities are in the adrenal and the midgestational chorion. Guinea pig gonads contain scarcely detectable activities. In CD-1 mice the highest specific activity is in testis and the highest total activity is in late placenta. Adrenals from both sexes and ovaries contain minimal activities, while liver and fetal membrane activities are remarkably low. In CD-1, DBA, C57BL, and BALB mice, qualitative patterns are similar. Purified or partially purified estrogen sulfotransferase from guinea pig adrenal and chorion were used to study the effect of a number of possible steroidal inhibitors. Considerable structural specificity is evident within a range of steroids, even among some which are not substrates. Pregnenolone is the most effective 21-carbon inhibitor and, in general, more highly hydroxylated forms are less inhibitory. Within a series of 21-, 19- and 18-carbon steroids, the structure of the A ring appears to be extremely important in regard to inhibitory effects.
豚鼠中雌激素磺基转移酶的总活性最高的部位是肝脏，而比活性最高的部位是肾上腺和妊娠中期的绒毛膜。豚鼠性腺中的活性几乎检测不到。在CD-1小鼠中，比活性最高的部位是睾丸，总活性最高的部位是晚期胎盘。两性的肾上腺和卵巢中的活性最低，而肝脏和胎膜的活性则非常低。在CD-1、DBA、C57BL和BALB小鼠中，定性模式相似。使用从豚鼠肾上腺和绒毛膜中纯化或部分纯化的雌激素磺基转移酶来研究多种可能的甾体抑制剂的作用。在一系列甾体中，即使在一些不是底物的甾体中，也明显存在相当程度的结构特异性。孕烯醇酮是最有效的21碳抑制剂，一般来说，羟基化程度更高的形式抑制作用较小。在一系列21碳、19碳和18碳甾体中，A环的结构在抑制作用方面似乎极其重要。
