在野外采集的活蚊子中检测疟原虫子孢子。

Detecting malaria sporozoites in live, field-collected mosquitoes.

作者信息

Habluetzel A, Merzagora L, Jenni L, Betschart B, Rotigliano G, Esposito F

机构信息

Department of Molecular, Cell, and Animal Biology, University of Camerino, Italy.

出版信息

Trans R Soc Trop Med Hyg. 1992 Mar-Apr;86(2):138-40. doi: 10.1016/0035-9203(92)90542-k.

DOI:10.1016/0035-9203(92)90542-k

PMID:1440771

Abstract

A method is described for identifying malaria-infected mosquitoes, without killing them or hampering their fitness. Individual mosquitoes were induced to salivate on coverslips, and sporozoites, deposited on the glass surface, were visualized by Giemsa staining. Of 21 mosquitoes found to contain sporozoites by salivary gland dissection, 13 had delivered sporozoites on coverslips. A positive correlation was found between the amount of saliva expelled and ejection of sporozoites, indicating that the sensitivity of the method may be increased by improving the probing behaviour of the mosquitoes. The procedure described may be suitable for selecting infected mosquitoes which are able to eject sporozoites during probing. Being applicable to wild Anopheles and to large numbers of mosquitoes, the method lends itself for use in field studies on malaria.

摘要

本文描述了一种鉴定感染疟疾蚊子的方法，该方法不会杀死蚊子或妨碍其健康。诱导单个蚊子在盖玻片上唾液分泌，沉积在玻璃表面的子孢子通过吉姆萨染色可视化。在通过唾液腺解剖发现含有子孢子的21只蚊子中，有13只在盖玻片上释放了子孢子。发现排出的唾液量与子孢子的排出之间存在正相关，这表明通过改善蚊子的刺探行为可以提高该方法的灵敏度。所描述的程序可能适用于选择在刺探过程中能够排出子孢子的感染蚊子。该方法适用于野生按蚊和大量蚊子，适用于疟疾的现场研究。