Beier J C, Onyango F K, Ramadhan M, Koros J K, Asiago C M, Wirtz R A, Koech D K, Roberts C R
Kenya Medical Research Institute, Nairobi.
Med Vet Entomol. 1991 Jan;5(1):63-70. doi: 10.1111/j.1365-2915.1991.tb00522.x.
The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).
通过显微镜检查确定了来自肯尼亚西部的1137只野生、自然感染的按蚊唾液腺中的疟原虫子孢子数量。感染性冈比亚按蚊复合组（n = 874）的子孢子几何平均数（GM）为962个，费氏按蚊（n = 263）为812个。在不同物种、采集技术或地点之间，子孢子的几何平均数未检测到显著差异。在感染性冈比亚按蚊中，1.7%（15/874）含有超过41,830个子孢子，这是观察到的费氏按蚊的最高数量。发现显微镜技术在检测唾液腺中低度子孢子感染方面比酶联免疫吸附测定（ELISA）更敏感。在1137例唾液腺感染中，83.6%的唾液腺子孢子通过ELISA鉴定为恶性疟原虫（n = 910）、卵形疟原虫（n = 7）、三日疟原虫（n = 3）或混合感染（n = 30）。ELISA无法鉴定的187例唾液腺感染所含子孢子明显少于可鉴定的感染（GM = 242对GM = 1200）。
