Nikonov A V, Korolev E V, Snigirevskaia E S, Brudnaia M S, Komissarchik Ia Iu, Ivanov P I, Borkhsenius S N
Tsitologiia. 1992;34(3):31-8.
In all the strains of M. gallisepticum investigated, a protein with apparent molecular weight 40 kDa was revealed by immunoblotting with polyclonal anti-calf brain tubulin antibodies and monoclonal anti-chicken alpha-tubulin antibodies. In other 8 investigated Mycoplasma species no positive reactions with the same antibodies were found. The M. Gallisepticum cells were examined under electron microscope on fine serial sections and on some sections going at different angles to the long cell axis. Undermembrane system of tubules was revealed and the intracellular pattern of the tubular structures were reconstructed. The immunoelectron microscopic data suggest that tubulin-like protein may be included into the structures.
在用多克隆抗小牛脑微管蛋白抗体和单克隆抗鸡α-微管蛋白抗体进行免疫印迹分析时,在所研究的所有鸡毒支原体菌株中,均发现了一种表观分子量为40 kDa的蛋白质。在其他8种所研究的支原体物种中,未发现与相同抗体的阳性反应。对鸡毒支原体细胞进行了电子显微镜检查,观察了精细的连续切片以及一些与细胞长轴成不同角度的切片。揭示了膜下微管系统,并重建了管状结构的细胞内模式。免疫电子显微镜数据表明,微管蛋白样蛋白可能包含在这些结构中。