[从小鼠血清中分离得到的铜蓝蛋白及其理化特性]
[Isolation and physico-chemical characteristics of ceruloplasmin obtained from murine serum].
作者信息
Livshits V I, Korol' D R, Lialiushko N M
出版信息
Vopr Med Khim. 1992 Jan-Feb;38(1):15-6.
PMID:1441284
Abstract
Ceruloplasmin was isolated from mice blood serum by means of ion exchange chromatography and gel filtration. Its content in blood was equal to 10-12 mg/100 ml, oxidase activity--9-12 U/mg of protein at E440, molecular mass--161.3 +/- 5.8 kDa. Properties of mice ceruloplasmin were studied as compared with the human and rat glycoprotein, isolated using the similar procedure.
摘要
通过离子交换色谱法和凝胶过滤法从小鼠血清中分离出铜蓝蛋白。其血液中的含量为10 - 12毫克/100毫升，氧化酶活性在E440下为9 - 12单位/毫克蛋白质，分子量为161.3±5.8千道尔顿。将小鼠铜蓝蛋白的性质与使用类似方法分离出的人及大鼠糖蛋白进行了比较研究。
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