A simple and rapid procedure for isolation of ceruloplasmin from porcine and bovine blood serum.

A rapid and simole procedure for isolation of ceruloplasmin from porcine and bovine blood sera was elaborated; it involves DEAE-Sephadex A-50 chromatography, precipitation with an ethanol-chloroform mixture plus extraction with saline, and preparative polyacrylamide-gel electrophoresis. The preparations obtained showed a high degree of electrophoretic homogeneity and a higher A610/A280 ratio and specific activity than the preparations obtained by the modified method of Broman & Kjellin (Biochem. Biophys. Acta, 82, 101-109, 1964).