Ling S S, Zhou Y, Sun W L, Zhu D C, Zhang H Y, Tang G Z, Jin Y F, Zhu J Z
Department of Pharmacology, General Hospital of Nanjing Command, PLA.
Yao Xue Xue Bao. 1992;27(6):412-7.
Erythropoietin (EPO) is a glycoprotein hormone secreted by human kidney cells. Human EPO was induced from human embryo kidney cells, isolated and purified from medium by biochemical method in our laboratory. The hypoproliferative anemia in chronic renal failure (CRF) has been assumed to be the result of decreased EPO production by the damaged kidney and of the shortening of the survival of erythrocytes. In this study, CRF anemia was formed 9 weeks after the removal of five-sixths of the renal mass of rats. These anemic rats were divided into 6 groups: treated with different dosages of EPO or physiological saline. The results indicate that EPO has apparent effects on anemia in rats with CRF. It may stimulate erythropoiesis and improve the anemia state of rats with CRF. Hematological parameters (RBC, Hb, PLT, Ht and Rt) may be reverted to normal levels (P less than 0.001). The level of BUN and Cr were significantly decreased. The optimum dose of EPO was 1000 U/kg. All these results show that injection of EPO has therapeutic effect on anemia in rats with CRF. EPO showed no effect on normal rats.
促红细胞生成素（EPO）是一种由人肾细胞分泌的糖蛋白激素。在我们实验室中，人促红细胞生成素是从人胚肾细胞诱导产生的，并通过生化方法从培养基中分离纯化得到。慢性肾衰竭（CRF）中的增生低下性贫血被认为是受损肾脏产生促红细胞生成素减少以及红细胞存活时间缩短的结果。在本研究中，大鼠肾切除五分之六后9周形成CRF贫血。将这些贫血大鼠分为6组：分别用不同剂量的促红细胞生成素或生理盐水进行治疗。结果表明，促红细胞生成素对CRF大鼠的贫血有明显作用。它可能刺激红细胞生成并改善CRF大鼠的贫血状态。血液学参数（红细胞计数、血红蛋白、血小板计数、血细胞比容和网织红细胞计数）可能恢复到正常水平（P小于0.001）。血尿素氮和肌酐水平显著降低。促红细胞生成素的最佳剂量为1000 U/kg。所有这些结果表明，注射促红细胞生成素对CRF大鼠的贫血有治疗作用。促红细胞生成素对正常大鼠无作用。
