Krumwieh D, Arnold I, Seiler F R
Research Laboratories of Behringwerke, Marburg/Lahn, W. Germany.
Behring Inst Mitt. 1988 Aug(83):193-201.
Highly purified, recombinant, human erythropoietin (rh EPO) has been compared to natural urinary derived erythropoietin (nEPO). Both EPO preparations have been characterized biologically: The proliferation of murine spleen cells in vitro after pretreatment with phenylhydrazine and the 59Fe incorporation into the heme of polycythemic mice have been determined. Further, the effect of rh EPO on the erythropoiesis of normal mice was studied. Depending on the applied rh EPO dosage an increase in hematocrit was observed. Treatment with rh EPO in rats with anemia due to subtotal nephrectomy also showed a dose-dependent rise of hematocrit and hemoglobin, and therefore a reversal of the anemic status. The comparison of rh EPO and natural EPO showed full biological activity of the recombinant protein and its equivalence to the natural hormone.
已将高度纯化的重组人促红细胞生成素（rh EPO）与天然尿源性促红细胞生成素（nEPO）进行了比较。两种促红细胞生成素制剂均已进行生物学特性鉴定：测定了用苯肼预处理后小鼠脾细胞在体外的增殖情况以及59Fe掺入多血质小鼠血红素中的情况。此外，还研究了rh EPO对正常小鼠红细胞生成的影响。根据所应用的rh EPO剂量，观察到血细胞比容增加。对因次全肾切除导致贫血的大鼠用rh EPO治疗也显示出血细胞比容和血红蛋白呈剂量依赖性升高，从而使贫血状态得到逆转。rh EPO与天然促红细胞生成素的比较表明重组蛋白具有完全的生物学活性且与天然激素等效。
