Yang D J, Zhang R
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1992;27(7):539-43.
The present paper describes a quantitative determination method of tetracyclines by reversed phase high performance liquid chromatography. The method used a Waters 10 C18 4.6 nm ID x 250 nm reversed phase column, with a mobile phase composed of 14% acetonitrile-3% dimethylformamide-83% water containing 0.02 mol/L citric acid (pH-2.5), the detector wavelength was set at 350 nm, and the flow rate was maintained at 1.0 ml/min. Demethylchlortetracycline, epi-demethylchlortetracycline, demethyltetracycline, epi-demethyltetracycline, tetracycline, epi-tetracycline, chlortetracycline and epi-chlortetracycline can be separated highly efficiently. The tetracyclines and their anhydro- products can be separated on the same column with 15% acetonitrile- 15% dimethylformamide- 70% 0.02 mol/L citric acid as the mobile phase, and detected at UV 270 nm.
本文描述了一种采用反相高效液相色谱法测定四环素的定量方法。该方法使用Waters 10 C18 4.6纳米内径×250纳米反相柱，流动相由含0.02摩尔/升柠檬酸（pH值为2.5）的14%乙腈 - 3%二甲基甲酰胺 - 83%水组成，检测波长设定为350纳米，流速保持在1.0毫升/分钟。去甲金霉素、表去甲金霉素、去甲四环素、表去甲四环素、四环素、表四环素、金霉素和表金霉素能够被高效分离。以15%乙腈 - 15%二甲基甲酰胺 - 70% 0.02摩尔/升柠檬酸为流动相时，四环素及其脱水产物可在同一根柱子上分离，并在紫外270纳米处进行检测。
