Taketani Y, Kuo T M, Mizuno M
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
Am J Obstet Gynecol. 1992 Jul;167(1):265-70. doi: 10.1016/s0002-9378(11)91672-x.
Our aim was to examine the relationship between the levels of cytokines in peritoneal fluid and its embryo toxicity.
The levels of interleukin-1 and tumor necrosis factor were measured in peritoneal fluid from infertile women who did not have endometriosis (n = 21), who had untreated endometriosis (n = 19), and who had undergone medical treatment for endometriosis (n = 10). Embryo toxicity was investigated in mouse two-cell embryos cocultured with the oviducts in culture media that contained various concentrations of peritoneal fluid.
The levels of cytokines were significantly higher in the peritoneal fluid from women who had untreated endometriosis than in women who did not have endometriosis, but they were extremely low in women who had undergone medical treatment with either danazol or buserelin. The peritoneal fluid from women who had untreated endometriosis adversely affected the cleavage of mouse two-cell embryos. After medical treatment the embryo toxicity of the peritoneal fluid was almost undetectable.
These results offer some theoretic bases in support of medical treatment to improve reproductive performance in infertile women who have endometriosis.
我们的目的是研究腹腔液中细胞因子水平与其胚胎毒性之间的关系。
测量了未患子宫内膜异位症的不孕妇女（n = 21）、未治疗的子宫内膜异位症患者（n = 19）以及接受过子宫内膜异位症药物治疗的患者（n = 10）腹腔液中的白细胞介素-1和肿瘤坏死因子水平。在含有不同浓度腹腔液的培养基中，与输卵管共同培养的小鼠二细胞胚胎中研究胚胎毒性。
未治疗的子宫内膜异位症患者腹腔液中的细胞因子水平显著高于未患子宫内膜异位症的妇女，但在用达那唑或布舍瑞林进行药物治疗的妇女中细胞因子水平极低。未治疗的子宫内膜异位症患者的腹腔液对小鼠二细胞胚胎的卵裂有不利影响。药物治疗后，腹腔液的胚胎毒性几乎检测不到。
这些结果为药物治疗改善患有子宫内膜异位症的不孕妇女的生殖性能提供了一些理论依据。
