OVERMAN J R, SHARP D G
J Exp Med. 1959 Sep 1;110(3):461-80. doi: 10.1084/jem.110.3.461.
Total virus particle counts, infectivity titrations and the ratios between particles and infective units have been determined for vaccinia virus infected tissues. Growth curves of vaccinia in the chorioallantoic membrane are characterized by relatively low ratios from 1 to 4 days after inoculation and a marked rise in the ratio at more prolonged intervals. Ratio determinations of vaccinia virus passages in the egg, rabbit skin, and guinea pig skin have been made to study the phenomenon of adaptation in different hosts. The embryonated egg chorioallantoic membrane shows no variation in the ratio of particles to infectious units during passage and it is concluded that this host is completely susceptible to vaccinia. During adaptive passages on the skin of rabbits and guinea pigs relatively large amounts of non-infective virus appear as indicated by a rise in the particle-infectivity ratios. The extent of ratio increase appears related to the general resistance of the host to the virus. Finally, treatment of crude tissue extracts with sonic vibration is described as an aid in dispersing the virus particles for quantitative particle counts.
已对感染痘苗病毒的组织进行了病毒颗粒总数、感染性滴定以及颗粒与感染单位之间的比率测定。痘苗在绒毛尿囊膜中的生长曲线的特征是,接种后1至4天比率相对较低,而在更长时间间隔时比率显著上升。已对痘苗病毒在鸡胚、兔皮肤和豚鼠皮肤中的传代进行了比率测定,以研究在不同宿主中的适应现象。在传代过程中,鸡胚绒毛尿囊膜的颗粒与感染性单位的比率没有变化,得出的结论是该宿主对痘苗完全易感。在兔和豚鼠皮肤的适应性传代过程中,如颗粒感染性比率的上升所示,出现了相对大量的非感染性病毒。比率增加的程度似乎与宿主对病毒的总体抵抗力有关。最后,描述了用超声振动处理粗组织提取物,以帮助分散病毒颗粒进行定量颗粒计数。